A comparative histological, histochemical and immunohistochemical study of the biocompatibility of three different nano-restorative materials implanted in rats’ connective tissue

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dc.contributor.author El-Rouby, Dalia H.
dc.contributor.author Abdel- Halim, Sahar A.
dc.date.accessioned 2018-02-26T14:40:42Z
dc.date.available 2018-02-26T14:40:42Z
dc.date.issued 2005
dc.identifier.citation Abdel- Halim, S. A. (2005). A comparative histological, histochemical and immunohistochemical study of the biocompatibility of three different nano-restorative materials implanted in rats’ connective tissue (Rep.). Egypt: Misr University for Science & Technology. en_US
dc.identifier.uri http://dspace.must.edu.eg/handle/123456789/544
dc.description.abstract This study was conducted to evaluate the subcutaneous connective tissue reaction to the newly-developed nano-restorative materials: Filtek Supreme XT, Ceram X and Ketac N100. These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of albino rats. Tissue biopsies were collected and histologically examined 1, 4 and 8 weeks after the implantation procedure. The presence of inflammation, type and location of inflammatory cells, calcification and fibrous tissue formation were recorded. Mast cells and macrophages were quantitatively assessed using toluidine blue stain and CD68 immunoexpression respectively. Microscopic analysis revealed that Ceram X and Filtek supreme XT produced an irritating effect only during the initial post implant period. Ketac N100 caused the severest irritation, producing an inflammatory reaction which ranged from moderate to intense over the entire experimental period. Comparing mast cell number and CD 68 area % between the control and the three tested materials, there was a significant difference throughout the experiment (p=0.000). The highest mast cell number was recorded in the Ketac N group after one week, while the greatest CD 68 immunoexpression was noted after 4 weeks in the same group. It can be concluded that the tested materials induce different and time dependent inflammatory reactions and that Ketac N 100 is less biocompatible compared to Filtek supreme NT and Ceram X. en_US
dc.language.iso en_US en_US
dc.publisher Misr University for Science & Technology en_US
dc.subject Biomaterials en_US
dc.subject METHODS en_US
dc.title A comparative histological, histochemical and immunohistochemical study of the biocompatibility of three different nano-restorative materials implanted in rats’ connective tissue en_US
dc.type Other en_US


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