Metabolome Classification of Brassica napus L. Organs via UPLC–QTOF–PDA–MS and Their Anti-oxidant Potential

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dc.contributor.author Farag, Mohamed A.
dc.contributor.author Sharaf Eldin, Mohamed G.
dc.contributor.author Kassem, Hanaa
dc.contributor.author Abou el Fetouha, Mohamed
dc.date.accessioned 2017-09-16T10:02:55Z
dc.date.available 2017-09-16T10:02:55Z
dc.date.issued 2012-09-16
dc.identifier.citation Farag, M. A., Sharaf Eldin, M. G., Kassem, H., & Abou el Fetouha, M. (2012). Metabolome Classification of Brassica napus L. Organs via UPLC–QTOF–PDA–MS and Their Anti-oxidant Potential. Wiley Online Library. doi:10.1002/pca.2408
dc.identifier.uri http://dspace.must.edu.eg/handle/123456789/258
dc.description.abstract Introduction – Brassica napus L. is a crop widely grown for its oil production and other nutritional components in the seed. In addition to the seed, other organs contain a wide range of phenolic metabolites although they have not been investigated to the same extent as in seeds. Objective – To define and compare the phytochemical composition of B. napus L. organs, namely the root, stem, leaf, inflorescence and seeds. Method – Non-targeted metabolomic analysis via UPLC–QTOF–MS was utilised in order to localise compounds belonging to various chemical classes (i.e. oxygenated fatty acids, flavonols, phenolic acids and sinapoyl choline derivatives). Results – The vast majority of identified metabolites were flavonol glycosides that accumulated in most of the plant organs. Whereas other classes were detected predominantly in specific organs, i.e. sinapoyl cholines were present uniquely in seeds. Furthermore, variation in the accumulation pattern of metabolites from the same class was observed, particularly in the case of quercetin, kaempferol and isorhamnetin flavonols. Anti-oxidant activity, based on 2,2-diphenyl-1-picrylhdrazyl analysis was observed for all extracts, and correlated to some extent with total flavonoid content. Conclusion – This study provides the most complete map for polyphenol composition in B. napus L. organs. By describing the metabolites profile in B. napus L., this study provides the basis for future investigations of seeds for potential health and/or medicinal use. Copyright © 2012 John Wiley & Sons, Ltd. en_US
dc.language.iso en en_US
dc.publisher Wiley Online Library en_US
dc.relation.ispartofseries phytochemical analysis;
dc.subject UPLC–MS en_US
dc.subject metabolite profiling en_US
dc.subject NMR en_US
dc.subject principal component analysis en_US
dc.subject anti-oxidant en_US
dc.subject flavonoids en_US
dc.subject Brassica napus L en_US
dc.title Metabolome Classification of Brassica napus L. Organs via UPLC–QTOF–PDA–MS and Their Anti-oxidant Potential en_US
dc.type Article en_US


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